Lateral flow assays

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Lateral flow assays

Lateral flow assays (LFAs) are the technology behind low-cost, simple, rapid and portable detection devices popular in biomedicine, agriculture, food and environmental sciences. This review presents an overview of the principle of the method and the critical components of the assay, focusing on lateral flow immunoassays. This type of assay has recently attracted considerable interest because of...

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Nanoparticle-based photoacoustic analysis for highly sensitive lateral flow assays.

This paper demonstrates a photoacoustics-based lateral flow test that takes advantage of the strong interaction of light and gold nanoparticles to quantitatively detect a disease biomarker. For a commercially available lateral flow test strip, the photoacoustic analysis improved the detection limit by two orders of magnitude compared to colorimetric measurements.

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Quantitative Lateral Flow Assays for Salivary Biomarker Assessment: A Review

Saliva is an emerging biofluid with a significant number of applications in use across research and clinical settings. The present paper explores the reasons why saliva has grown in popularity in recent years, balancing both the potential strengths and weaknesses of this biofluid. Focusing on reasons why saliva is different from other common biological fluids such as blood, urine, or tears, we ...

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Analytical Tools to Improve Optimization Procedures for Lateral Flow Assays

Immunochromatographic or lateral flow assays (LFAs) are inexpensive, easy to use, point-of-care medical diagnostic tests that are found in arenas ranging from a doctor's office in Manhattan to a rural medical clinic in low resource settings. The simplicity in the LFA itself belies the complex task of optimization required to make the test sensitive, rapid and easy to use. Currently, the manufac...

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Multiplex Flow Assays

Lateral flow or dipstick assays (e.g., home pregnancy tests), where an analyte solution is drawn through a porous membrane and is detected by localization onto a capture probe residing at a specific site on the flow strip, are the most commonly and extensively used type of diagnostic assay. However, after over 30 years of use, these assays are constrained to measuring one or a few analytes at a...

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ژورنال

عنوان ژورنال: Essays in Biochemistry

سال: 2016

ISSN: 0071-1365,1744-1358

DOI: 10.1042/ebc20150012